Page 7 - Analityka 2/2015

Retention mechanism NAUKA
in high performance liquid
chromatography

PART II: REVERSED PHASE LIQUID CHROMATOGRAPHY

The variety of compounds present in the world around us necessitates adjustment of the optimal analytical param-
eters for a given substance, which allow for efficient analysis of high selectivity and resolution. High performance
liquid chromatography in reversed phase is a convenient method to analyze non-polar compounds. In reversed
phase system the stationary phase is less polar than the mobile phase. As the stationary phase the chemically
modified silica is used among the octyl, octadecyl, phenyl-propyl, cholesterol groups. The mobile phase is a mix-
ture of water and an organic solvent, usually methanol and acetonitrile. In this system, the retention mechanism is
twofold. The retention of solute`s correspond to the phenomenon of the adsorption and or the partition between
the mobile phase and the stationary phase. High performance liquid chromatography in reversed phase has the
widest application in separation techniques.

ANALITYKA – NUMER 2, ROK (XVI) 2015 5